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Virology
Virology physician and researcher
Peter Hotez, MD, should be terminated from services on or after May 30, 2022.

Molecular Virology & Microbiology

Did he answer all relevant virology questions on or before May 20, 2022?
No

Is continuing in this job on or after May 30, 2022, justified?
No.

Virology physician and researcher

What is a serotype?
Serotypes refer to separate groups within a species of microorganisms that all share a similar characteristic.

Are you are virologist?

Are you ready to answer questions relevant to your job?

What were the causes of the 2019 to 2022 viral pandemic?

Is this a mutated influenza virus, rhinovirus, picornavirus, adenovirus, human respiratory syncytial virus, enterovirus, or human coronavirus with serotypes?

How do you scientifically prove it is a mutated influenza virus, rhinovirus, picornavirus, adenovirus, human respiratory syncytial virus, enterovirus, or human coronavirus with serotypes?

What are the respiratory problems associated with most of the viruses?
These may include coughing, sore throat, runny nose, sneezing, headache, and fever.

How are allergic rhinitis, bronchitis, bronchiolitis, pertussis, sinusitis, and upper respiratory track viral infection different?

There are at least 200 virus strains implicated in causing the common cold, with rhinoviruses, coronaviruses, adenoviruses, and enteroviruses being the most common.

If existing individuals claiming to be virologists answer these questions, they should show where they have answered these questions. They must resign on or after May 30, 2022, if they are not able to answer these questions.

Matters related to enlisting all entities who received grants for virology and how they answered these questions.
Stop their grants on or after May 30, 2022, for failing to answer questions relevant to their jobs.

Vaccine Issues
Here are guidelines from Doctor Asif Qureshi.
What must the head of the state healthcare know about a vaccine?
  1. Annotation or definition

  2. Types of vaccines

  3. Types of microscopes

  4. Measurements that scientists, researchers, and manufacturers of a vaccine must know.

  5. Available vaccines

  6. New vaccine for a specific virus

  7. Vaccine Issues
All this is part of the research process.

Annotation or definition
What is a vaccine?
How do vaccines work?
What is vaccination?
What is immunization?
Why is immunization important?
How effective are vaccines?
How safe are vaccines?
Where to get immunized
When to get immunized
Children's Vaccination: What is the schedule?
When was it last updated?
Who updated it?
How do vaccines work?
Are there any vaccine side-effects?
Why is vaccinating so important? What are the ingredients / additives of vaccines?
What vaccines do adults need?
What vaccines do children need? What diseases do vaccines prevent?
What are some of the common misconceptions about vaccinating?
What would happen if we stopped immunizations?
Does your pharmacy give shots?
How do you know vaccines are genuine?
How do you know vaccines won't cause harm?
How do you know it's not a placebo?
How do you confirm genuineness?
What medical history is necessary before a vaccine is adminstered?
What follow-up is necessary to measure the effectiveness of a vaccine?
What are the various vaccines available in 2012?
How are vaccines manufactured?
How is the effectiveness of a vaccine verified?
What are the dose, age and route of administration of each vaccine?
Which vaccine is administered in left or right deltoid?
Which vaccine is administered orally?
What is a vaccine?
A vaccine is a substance that primes the body’s immune system to make antibodies, T-cells and memory cells which are the body’s defense against infection. When you are vaccinated you actually build up your immune system, making you stronger and more resistant to disease as you grow. Vaccines are the best way to protect you and your family against some very serious infections.

How do vaccines work?
A vaccine contains a killed or weakened part of a germ that is responsible for infection. Because the germ has been killed or weakened before it is used to make the vaccine, it can not make the person sick. When a person receives a vaccine, the body reacts by making protective substances called "antibodies". The antibodies are the body's defenders because they help to kill off the germs that enter the body. In other words, vaccines expose people safely to germs, so that they can become protected from a disease but not come down with the disease.

What is vaccination?
Vaccination means having the vaccine - actually getting the injection.

What is immunization?
Immunization means both receiving the vaccine and becoming immune to ward off a disease as a result of immunization.

Like eating well and exercise, getting immunized is a foundation for a healthy life.

Immunizations help save lives, prevent serious illnesses, and are recognized as one of the most effective public health interventions. Immunizations help the body make its own protection (or antibodies) against certain diseases.

Why is immunization important?
When children are immunized, their bodies make antibodies that fight specific infections. If they are not protected and come in contact with one of these infections, they may get very sick and potentially experience complications, or even die.

How effective are vaccines?
Vaccines are very effective in preventing disease when given as recommended. However, no vaccine will work for 100 per cent of the children who receive it. Studies of disease outbreaks show that although some immunized children can develop the infection, the illness is often less severe.

How safe are vaccines?
All vaccines have to be tested to make sure they are both safe and effective. The most common side effects are mild pain, swelling and redness where the injection was given.

Some infant vaccines may cause a low-grade fever (approximately 38°C) or fussiness for a day or two after the injection. Physicians may recommend acetaminophen to prevent fever and pain. Serious side effects from immunizations are rare. Please report any side effects or severe vaccine reactions to your health care provider or local public health unit. You should always discuss the benefits and risks of any vaccine with your health care provider.

Children's Vaccination: What is the schedule?
When was it last updated?
Who updated it?

Your child should get the first shots at 2 months of age (or in some cases before leaving the hospital after birth), then at 4 months, 6 months and 12-15 months of age. Remember, each of these visits is important! Your child must complete the series to be fully protected. More immunizations are recommended at 2 years and before school entry. Adolescents also need immunizations at 11-12 years of age, before entering 7th grade.

2 months old
4 months old
6 months old
12 months old
15 months old
18 months old
4 - 6 years old
Grade 7 students
Grade 8 females
14 -16 years old (10 years after 4-6 year old booster)
Every year (in autumn)

Vaccines that protect against the following diseases are available free of charge, and are required for attendance at school (unless there is a valid written exemption) :

•Diphtheria is a very serious bacterial infection. It can cause breathing problems, heart failure, nerve damage and death in about 10% of cases.

•Tetanus (Lockjaw) causes painful muscle spasms, breathing failure and can lead to death. It is caused by bacteria and spores in the soil that can infect wounds.

•Polio can cause paralysis (loss of control over muscles in the body), inflammation of the brain and death. People get polio from drinking water or eating food with the polio virus in it. It is no longer common in Canada because of high immunization rates, but cases do occur elsewhere in the world and polio may be acquired when traveling if you are not fully immunized.

•Measles causes rash, high fever, cough, runny nose and watery eyes. It can cause middle ear infection, pneumonia (lung infection), inflammation of the brain, hearing loss, brain damage and death.

•Mumps causes fever, headache, painful swelling of the glands in the mouth and neck, earache and can cause inflammation of the brain. It can cause temporary or permanent deafness and swelling of the ovaries in women and testes in men, possibly leading to sterility.

•Rubella (German Measles) causes fever, rash, swelling of the neck glands and swelling and pain in the joints. It can cause bruising and bleeding. If a pregnant woman gets rubella, it can be very dangerous for the unborn baby.

Vaccines against the following diseases are recommended but not required for attendance at school. These vaccines are available free of charge :

•Pertussis (Whooping Cough) causes severe coughing spells for weeks or months. It can also cause pneumonia (lung infection), middle ear infection, convulsions (seizures), inflammation of the brain and death. The risk of complications is greatest in children younger than one year of age.

•Hepatitis B is a virus that can cause serious liver problems that can be fatal, such as liver failure and liver cancer. The vaccine is free for grade 7 students and certain high-risk groups (including infants born to mothers who are infected with hepatitis B and can pass the disease on to their babies).

•Influenza (the Flu) is a viral infection that causes cough, high fever, chills, headache and muscle pain. It can cause pneumonia (infection of the lungs), middle ear infections, heart failure and death. The danger of this infection varies from year to year depending on the strain and can be mild to life-threatening.

•Varicella (Chickenpox) is a highly contagious viral infection. It can cause fever, headache, chills, muscle or joint aches a day or two before the itchy, red rash appears. A pregnant woman with chickenpox can pass it on to her unborn baby. Mothers with chickenpox can also give it to their newborn baby after birth. Chickenpox can be very severe or even life-threatening to newborn babies.

•Meningococcal disease is a very serious bacterial infection and a common cause of meningitis (infection of the lining of the brain and spinal cord) and meningococcaemia (severe infection of the blood) that can cause severe complications and death.

•Human Papillomavirus (HPV) is a very common virus transmitted through sexual activity. HPV has been found to cause cervical cancer, some other rare cancers and genital warts. (About 75 per cent of adults will have at least one HPV infection in their lifetime.) The vaccine is free for grade 8 females.

Vaccines against the following diseases are recommended for younger children. These vaccines are available free of charge :

•Rotavirus is one of the leading causes of severe diarrhea in infants and children. Rotavirus is a very common and is easily spread from person to person. Rotavirus causes inflammation of the stomach and intestines (gastroenteritis). This vaccine is recommended for infants between 6 to 24 weeks of age.

•Haemophilus Influenzae type b (HIB) is a bacteria that can infect any part of the body. It can cause middle ear infections, breathing problems, damage to joints, pneumonia (lung infection), inflammation of the brain leading to brain damage and death. This vaccine is recommended for children less than 5 years of age.

•Pneumococcal disease is a bacterial infection that can cause serious illnesses such as pneumonia, blood infection and meningitis. The pneumococcal conjugate vaccine is now available free of charge in Ontario for the routine immunization of children less than 2 years old as well as high-risk children 2 to 59 months of age.

Because of changes in the influenza (flu) strains, adults also need to receive the flu shot each year.

Adults should continue to get the tetanus and diphtheria vaccine every 10 years throughout life, to be protected against these diseases.

Thinking about getting pregnant?

Be sure you are protected against rubella before pregnancy to protect your future baby from serious problems during its development.

Vaccinations/Injections
Vaccines
Vaccine
    How are vaccines manufactured?
Adult Vaccines
    Do you know the facts about adult vaccines?
Signs and symptoms of flu
    What is influenza (flu)?
    What are the symptoms of the flu?

    Here are further guidelines.
Flu Vaccine
    What is it?
    What are its constituents?
    Who manufactured these Flu Shots?
    How will this help to prevent Flue?
    Have there been reports of flue after Flue Shots?
    How is flu vaccine manufactured?
    How is the effectiveness of flu vaccine measured?
    Here are further guidelines.

Types of vaccines
What are the various categories of vaccines?
Live-attenuated vaccines
Inactivated vaccines
Subunit, recombinant, polysaccharide, and conjugate vaccines
Toxoid vaccines

Live-attenuated vaccines: What are various examples?
Inactivated vaccines: What are various examples?
Subunit, recombinant, polysaccharide, and conjugate vaccines: What are various examples?
Toxoid vaccines: What are various examples?


Available vaccines

What vaccines are available in the United States as of November 24, 2020?
1. Adenovirus
2. Anthrax
3. Cholera
4. Diphtheria
5. Hepatitis
6. Hepatitis B
7. Haemophilus influenzae type b (Hib)
8. Human Papillomavirus (HPV)
9. Seasonal Influenza (Flu) only
10. Japanese Encephalitis (JE) (Ixiaro)
11. Measles
12. Meningococcal
13. Mumps
14. Pertussis
15. Pneumococcal
16. Polio
17. Rabies
18. Rotavirus
19. Rubella
20. Shingles
21. Smallpox
22. Tetanus
23. Tuberculosis
24. Typhoid
25. Varicella
26. Yellow Fever

Asia-Pacific

The program now consists of vaccination for 12 diseases- tuberculosis, diphtheria, pertussis (whooping cough), tetanus, poliomyelitis, measles, hepatitis B, diarrhoea, Japanese encephalitis, rubella, pneumonia (haemophilus influenzae type B) and Pneumococcal diseases (pneumococcal pneumonia and meningitis).

Types of microscopes
What are the types of microscopes?
What is a magnification power?
What is a light microscope?
What is an electron microscope?


What are the types of microscopes?
Optical microscope
Electron microscope
Scanning probe microscope (SPM)
Others

Optical microscope

Binocular stereoscopic microscope
Brightfield microscope
Polarizing microscope
Phase contrast microscope
Differential interference contrast microscope
Fluorescence microscope
Total internal reflection fluorescence microscope
Laser microscope (Laser scanning confocal microscope)
Multiphoton excitation microscope
Structured illumination microscope
Main Types of Microscopes

The table below describes the main types of microscopes within the optical, electron, and scanning probe categories.

Optical microscope

Type Description
Binocular stereoscopic microscope A microscope that allows easy observation of 3D objects at low magnification.
Brightfield microscope A typical microscope that uses transmitted light to observe targets at high magnification.
Polarizing microscope A microscope that uses different light transmission characteristics of materials, such as crystalline structures, to produce an image.
Phase contrast microscope A microscope that visualizes minute surface irregularities by using light interference. It is commonly used to observe living cells without staining them.
What is a phase contrast microscope?

With a conventional biological microscope, it is difficult to observe colorless, transparent cells while they are alive. A phase contrast microscope makes it possible by utilizing two characteristics of light, diffraction and interference, to visualize specimens based on brightness differences (contrast).

Principle
With regard to periodic movements, such as sinusoidal waves, the phase represents the portion of the wave that has elapsed relative to the origin. Light is also an oscillation and the phase changes, when passing through an object, between the light that has passed through (diffracted light) and the remaining light (direct light). Even if the object is colorless and transparent, there is still a change in phase when light pass through it. This phase contrast is converted into brightness differences to observe specimens.
Features
  • Transparent cells can be observed without staining them because the phase contrast can be converted into brightness differences.
  • Because it is not necessary to stain cells, cell division and other processes can be observed in a living state.
Structure
Because diffracted light is too weak to be normally observed by the eye, a phase plate is located at the focal point of light between the objective lens and the image surface so that only the phase of the direct light changes. This generates contrast on the image surface.
Structural features include a ring aperture, instead of a pinhole, on the focal plane of the converging lens and a phase plate on the rear focal plane of the objective lens.
Differential interference contrast microscope This microscope, similar to the phase contrast, is used to observe minute surface irregularities but at a higher resolution. However, the use of polarized light limits the variety of observable specimen containers.
Fluorescence microscope A biological microscope that observes fluorescence emitted by samples by using special light sources such as mercury lamps. When combined with additional equipment, brightfield microscopes can also perform fluorescence imaging.
What is a fluorescence microscope?

A fluorescence microscope enables cells and proteins to be observed by using a fluorescent protein or antibody as a label. This type of microscope is indispensable for modern cell biology.

Principle
Fluorescent materials absorb a specific wavelength of light (excitation light) and emit light of a longer wavelength (fluorescence), which is based on Stokes’ law. For example, the fluorescence emitted by a target molecule can be observed by adding a specific fluorescent reagent to cells and then applying excitation light. A fluorescent microscope has all of the components to induce this fluorescence and capture the resulting image.
Features
  • Allows observation of fluorescence images, in addition to observation with transmitted light.
  • It is possible to only observe specific areas by using different fluorescent labels.
  • Fluorescent dyes enable users to view localization of particular proteins in cells.
  • The use of fluorescent proteins such as GFP allows for observation of living cells.
Structure
Generally, a fluorescence microscope is a combination of a biological microscope and fluorescent incident illumination equipment. The structure includes a focusing knob, an XY stage handle for positioning specimens, and a revolver for switching objective lenses. For lighting, it is also equipped with a cube turret that adjusts the wavelength of the excitation light, a shutter that prevents photobleaching of samples, and an ND (neutral density) filter that adjusts the strength of the excitation light.
Total internal reflection fluorescence microscope A fluorescence microscope that uses an evanescent wave to only illuminate near the surface of a specimen. The region that is viewed is generally very thin compared to conventional microscopes. Observation is possible in molecular units due to reduced background light.
Laser microscope
(Laser scanning confocal microscope)
This microscope uses laser beams for clear observation of thick samples with different focal distances.
What is a laser scanning confocal microscope?

This type of microscope is characterized by using laser beams as the light source. Laser scanning allows high-resolution observation as well as accurate 3D measurement.

Principle
These systems scan the surface of an object with a laser(s), record the spatial distribution of fluorescence and reflected light from the focal plane, and then visualize the resulting data with a computer to allow observation of high-resolution images.
As the name implies, this microscope uses a confocal optical system.
Features
  • By scanning the laser across the surface of an object in the X, Y, and Z planes, a high-resolution image with corresponding height data can be captured. With a biological sample, for example, this allows users to understand its 3D structure as well as to obtain clear fluorescence images.
  • While general optical microscopes use an image-forming optical system, laser microscopes use the confocal optical system. The former illuminates a specific area entirely while the latter focuses light on a single point with a point source light. Furthermore, a pinhole is provided at the image position to receive only the focused light. This results in better contrast with no unnecessary scattered light entering from the surrounding areas.
Structure
Laser beams emitted from the laser light source go through the objective lens to scan a sample. The fluorescence of the sample excited by the laser beams is returned to the objective lens, processed as an image, filtered by the pinhole, and displayed on the monitor.
Multiphoton excitation microscope The use of multiple excitation lasers reduces damage to cells and allows high-resolution observation of deep areas. This type of microscope is used to observe nerve cells and blood flow in the brain.
Structured illumination microscope A high-resolution microscope with advanced technology to overcome limited resolution found in optical microscopes that is caused by the diffraction of light.
What is a structured illumination microscope?

A type of high-resolution microscope based on technology that has overcome the limited resolution of optical microscopes caused by the diffraction limit of light.

Principle
Conventionally, the resolution of optical microscopes was limited to 200 nm or larger due to the diffraction limit of light. This limit has been overcome by a high-resolution microscope developed in the United States that is based on structured illumination. Structured illumination microscopy enables high-resolution images to be obtained by using the moire effect of a grid or other patterned illumination (structured illumination) to capture diffracted light, which is impossible with conventional optical microscopes.
Features
  • Provides much higher resolution than conventional optical microscopes, approximately twofold, both in the horizontal and vertical directions.
  • Ability to process multiple captured images at high speed makes live imaging of cells possible.
Structure
Structured illumination microscopes do not have a new structure but use a new way to capture light. More specifically, this type of microscope is based on moire fringes, which are caused by interference of light, and is designed to emit a specific pattern of light (structured illumination) to generate moire effects. Because images captured through this technology contain detailed information about the object, high-resolution images can be composed through computerized analysis of multiple images.

Electron microscope

Type Description
Transmission electron microscope (TEM), scanning electron microscope (SEM), etc. These microscopes emit electron beams, not light beams, toward targets to magnify them.

Scanning probe microscope (SPM)

Type Description
Atomic force microscope (AFM), scanning near-field optical microscope (SNOM), etc. This microscope scans the surface of samples with a probe and this interaction is used to measure fine surface shapes or properties.

Others

Type Description
X-ray microscope, ultrasonic microscope, etc.

Digital Microscopes

Digital Microscope System Leica DMS300

The Leica DMS300 is a complete digital microscope system that utilizes a HDMI-monitor instead of eyepieces. Leica’s high quality 8:1 zoom optics are combined with a 2.5MP camera to provide a full high-definition live image with up to 30fps. The Leica DMS300 produces high-quality, full-color still images as well as Full-HD movies as a standalone system.

All primary functions of the DMS300 are controlled with a wireless remote control. Additionally, it can be connected to a computer offering full compatibility with Leica software LAS EZ. The dual live video stream allows for simultaneous output to a PC and a second imaging device with HDMI-interface such as a HD-monitors or HD-projector.

Digital Microscope
VHX-7000 Series
-
The Different Types of Microscopes

Exploring the Top Four and More

There are several different types of microscopes used in light microscopy, and the four most popular types are Compound, Stereo, Digital and the Pocket or handheld microscopes.

Some types are best suited for biological applications, where others are best for classroom or personal hobby use.

Outside of light microscopy are the exciting developments with electron microscopes and in scanning probe microscopy.

The Compound Light Microscope

Commonly binocular (two eyepieces), the compound light microscope, combines the power of lenses and light to enlarge the subject being viewed.

Typically, the eyepiece itself allows for 10X or 15X magnification and when combined with the three or four objective lenses, which can be rotated into the field of view, produce higher magnification to a maximum of around 1000X generally.

The compound light microscope is popular among botanists for studying plant cells, in biology to view bacteria and parasites as well as a variety of human/animal cells.

It is a useful microscope in forensic labs for identifying drug structures.

Compound light microscopes are one of the most familiar of the different types of microscopes as they are most often found in science and biology classrooms.

For this reason, simple models are readily available and are inexpensive.

As well, several microscopy imaging techniques benefit scientists and researchers using the compound microscope and are worth exploring.

The Stereo Microscope

The Stereo microscope, also called a dissecting microscope, has two optical paths at slightly different angles allowing the image to be viewed three-dimensionally under the lenses.

Stereo microscopes magnify at low power, typically between 10X and 200X, generally below 100x.

With this type of microscope you generally have the choice of purchasing the fixed or zoom variety from a manufacturer and are relatively inexpensive.

Uses for this type of microscope include looking at surfaces, microsurgery, and watch making, plus building and inspecting circuit boards.

Stereo microscopes allow students to observe plant photosynthesis in action.

The Digital Microscope

Step into the 21st century with a digital microscope and enter a world of amazing detail.

The digital microscope, invented in Japan in 1986, uses the power of the computer to view objects not visible to the naked eye.

Among the different types of microscopes, this kind can be found with or without eyepieces to peer into.

It connects to a computer monitor via a USB cable, much like connecting a printer or mouse. The computer software allows the monitor to display the magnified specimen. Moving images can be recorded or single images captured in the computer’s memory.

An advantage of digital microscopes is the ability to email images, as well as comfortably watch moving images for long periods.

The USB Computer Microscope

Although not well suited to the same scientific applications as other light microscopes, the USB Computer microscope, among the different types of microscopes, can be used on almost any object and requires no preparation of the specimen.

It is essentially a macro lens used to examine images on a computer screen plugged into its USB port.

However, the magnification is restricted and is not comparable to your standard compound light microscope at only up to 200X with a relatively small depth of field.

The Pocket Microscope

In examining the different types of microscopes available on the market, the pocket microscope may be tiny but its abilities are impressive.

This is a device which is a great gift for a child or your student. It is used by scientists for hand-held imaging of a variety of specimens/objects in the field or in the laboratory.

It is small, durable and portable with a magnification ranging from 25x to 100x. There are many different models available.

You may even want to check out the portable digital microscopes that are available now as this is an invaluable tool to aid in image sharing and analysis.

The Electron Microscope
v Among the different types of microscopes, the Electron Microscope(EM) is a powerful microscope available and used today, allowing researchers to view a specimen at nanometer size.
v The transmission electron microscope(TEM), the first type of EM, is capable of producing images 1 nanometer in size.

The TEM is a popular choice for nanotechnology as well as semiconductor analysis and production.

A second type of electron microscope is the scanning electron microscope(SEM)are approximately 10 times less powerful than TEMs, they produce high-resolution, sharp, black and white 3D images.

The Transmission Electron Microscopes and Scanning Electron Microscopes have practical applications in such fields as biology, chemistry, gemology, metallurgy and industry as well as provide information on the topography, morphology, composition and crystallographic data of samples.

The Scanning Probe Microscope (SPM)

Among the different types of microscopes and microscopy techniques, scanning probe microscopy is used today in academic and industrial settings for those sectors involving physics, biology and chemistry. These instruments are used in research and development as standard analysis tools.
v Images are highly magnified and are observed as three-dimensional-shaped-specimens in real time. SPMs employ a delicate probe to scan the surface of the specimen eliminating the limitations that are found in electron and light microscopy.

The Acoustic Microscope

The Acoustic Microscope is less about resolution and more about finding faults, cracks or errors from samples during the manufacturing process.

With the use of high ultrasound, this type of microscope is the easiest intra-cavity imaging tool available. It is a microscope that is under used primarily due to the fact that it is less known for its capabilities.

Scanning acoustic microscopy, or SAM, is the most current type of acoustic microscopy available to today's scientists. They can use it to view a sample internally without staining it or causing it any damage thanks to point focusing technology, which relies on a beam to scan and penetrate the specimen while it is in water.

What is a light microscope?
•A light microscope is a biology laboratory instrument or tool, that uses visible light to detect and magnify very small objects, and enlarging them.
•They use lenses to focus light on the specimen, magnifying it thus producing an image. The specimen is normally placed close to the microscopic lens.
Microscopic magnification varies greatly depending on the types and number of lenses that make up the microscope. Depending on the number of lenses, there are two types of microscopes i. e Simple light microscope (it has low magnification because it uses a single lens) and the Compound light microscope (it has a higher magnification compared to the simple microscope because it uses at least two sets of lenses, an objective lens, and an eyepiece). The lenses are aligned in that, they can be able to bend light for efficient magnification of the image.
•The functioning of the light microscope is based on its ability to focus a beam of light through a specimen, which is very small and transparent, to produce an image. The image is then passed through one or two lenses for magnification for viewing. The transparency of the specimen allows easy and quick penetration of light. Specimens can vary from bacterial to cells and other microbial particles
Types of light microscopes (optical microscope)
The modern types of Light Microscopes include:
1.Bright field Light Microscope
2.Phase Contrast Light Microscope
3.Dark-Field Light Microscope
4.Fluorescence Light Microscope

What is an electron microscope?
Electron microscopy (EM) is a technique for obtaining high resolution images of biological and non-biological specimens.

An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects. A scanning transmission electron microscope has achieved better than 50 pm resolution in annular dark-field imaging mode and magnifications of up to about 10,000,000× whereas most light microscopes are limited by diffraction to about 200 nm resolution and useful magnifications below 2000×.

How do electron microscopes work?
If you've ever used an ordinary microscope, you'll know the basic idea is simple. There's a light at the bottom that shines upward through a thin slice of the specimen. You look through an eyepiece and a powerful lens to see a considerably magnified image of the specimen (typically 10–200 times bigger). So there are essentially four important parts to an ordinary microscope:

The source of light.
The specimen.
The lenses that makes the specimen seem bigger.
The magnified image of the specimen that you see.
In an electron microscope, these four things are slightly different.

The light source is replaced by a beam of very fast moving electrons.
The specimen usually has to be specially prepared and held inside a vacuum chamber from which the air has been pumped out (because electrons do not travel very far in air).
The lenses are replaced by a series of coil-shaped electromagnets through which the electron beam travels. In an ordinary microscope, the glass lenses bend (or refract) the light beams passing through them to produce magnification. In an electron microscope, the coils bend the electron beams the same way.
The image is formed as a photograph (called an electron micrograph) or as an image on a TV screen.
That's the basic, general idea of an electron microscope. But there are actually quite a few different types of electron microscopes and they all work in different ways. The three most familiar types are called transmission electron microscopes (TEMs), scanning electron microscopes (SEMs), and scanning tunneling microscopes (STMs).

Applications

Semiconductor and data storage
Circuit edit
Defect analysis
Failure analysis

Biology and life sciences
Cryobiology
Cryo-electron microscopy
Diagnostic electron microscopy
Drug research (e.g. antibiotics)
Electron tomography
Particle analysis
Particle detection
Protein localization
Structural biology
Tissue imaging
Toxicology
Virology (e.g. viral load monitoring)
Materials research
Device testing and characterization
Dynamic materials experiments
Electron beam-induced deposition
In-situ characterisation
Materials qualification
Medical research
Nanometrology
Nanoprototyping

Other
Chemical/Petrochemical
Direct beam-writing fabrication
Food science
Forensics
Fractography
Micro-characterization
Mining (mineral liberation analysis)
Pharmaceutical QC

Measurements that scientists, researchers, and manufacturers of a vaccine must know.
International System of Units (Metric System)
Dimension Common Unit Symbol American Equivalent
Length Meter m 39 inches
Mass Gram 0.033 ounces
Volume Liter L 1.06 quarts

How do you measure human cells, bacteria, viruses, and atoms?
Start with millimeters, micrometers, nanometers, and picometers.
Measure bacteria in micrometers
Measure a virus in nanometers
Measure atoms in picometers

1 meter = 100 centimeters
1 meter = 1000 millimeters
1 centimeter = 10 millimeters
1 millimeter = 1000 micrometers
1 micrometer = 1000 nanometers
1 nanometer = 1000 picometers

What is the size of human cells, bacteria, viruses, and atoms?
Human cell diameter = 25 micrometers or 25,000 nanometers
Cell nucleus (control center of cell) diameter = 5 micrometers or 5,000 nanometers
Bacteria cell length = 2 micrometers or 2,000 nanometers
Mitochondria (powerhouse of cell) length = 2 micrometers or 2,000 nanometers
Ribosome diameter (protein factory in cell) = 0.03 micrometers or 30 nanometers
Cell membrane thickness = 0.08 micrometers or 80 nanometers
Silver nanoparticle diameter = 0.04 micrometers or 40 nanometers

Atomic size, atomic mass, atomic number: What is the difference?
Atomic size: Picometers are used to measure the size of atomic structures.
Atomic number: Take a look at this: https://www.qureshiuniversity.com/elements1.html
Atomic mass: Take a look at this: https://www.qureshiuniversity.com/atomicmass.html

How many oxygen atoms lined up in a row would fit in a one nanometer space?
Seven.

What is the diameter of one oxygen atom?
The diameter of one oxygen atom is approximately 0.14 nanometers

What is the diameter of one hydrogen atom?
You can look up the covalent radius of hydrogen (37 pm) and double it for the diameter.
Helium has the smallest atomic radius.

What are some other useful links for vaccines?
If you know any other useful resources for vaccines, email admin@qureshiuniversity.com

New vaccine for a specific virus
How do you make a new vaccine for a specific virus?
Get answers to all questions relevant to the specific virus.
Decide the category of vaccines by manufacturer that needs to be manufactured.
Verify safety and effectiveness of the new vaccine.
No question relevant to a new vaccine for a specific virus can remain unanswered.

Vaccine manufacture and approval for human use process.
Questions that need to be answered.
https://qureshiuniversity.com/healthcarelaws.html#Vaccine manufacture and approval for human use process.

Vaccine manufacture and approval for human use process.
2019–2020 viral pandemic vaccine
Who all came forward to manufacture this specific vaccine?
Have they answered all questions relevant to the virus?
Have all the findings been proven using scientific methods?
What are the electron microscopic findings related to the virus?
Who revealed the electron microscopic findings related to the virus?
Who verified these findings?
How will they scientifically differentiate among influenza, rhinovirus, and coronavirus?
There are 10 patients presenting with a fever, cough, and sore throat. A few have influenza, a few have rhinovirus, and others have coronavirus. What tests will you run to prove the existence of a specific virus?
Who received grants for this vaccine without answering these questions on or before November 22, 2020?
Was it necessary to verify these findings before approving grants for the vaccine?
Is it justified?
Is the vaccine safe for human beings?
Is the vaccine effective for human beings?
Should the mentioned vaccine be approved for human use on or after November 22, 2020?
What evidence proves the safety and efficacy of the mentioned vaccine?
Have they answered all questions relevant to this vaccine?
Here are further guidelines.

Vaccine Issues
New vaccine approval process: What are various findings?
On or before December 2, 2020, the Medicines and Healthcare products Regulatory Agency (MHRA) at 10 South Colonnade, London, did not get relevant questions answered before approving the vaccine for coronavirus.

What must happen next?
An interview with Vanessa Birchall-Scott, Director of Human Resources Medicines and Healthcare products Regulatory Agency (MHRA), 10 South Colonnade, London December 2, 2020.

What questions do they need to answer?
Is it correct that your resource has approved the coronavirus vaccine?
Have they answered all questions relevant to the virus?
Have all the findings been proven using scientific methods?
What are the electron microscopic findings related to the virus?
Who revealed the electron microscopic findings related to the virus?
Who verified these findings?
How will they scientifically differentiate among influenza, rhinovirus, and coronavirus?
There are 10 patients presenting with a fever, cough, and sore throat. A few have influenza, a few have rhinovirus, and others have coronavirus. What tests will you run to prove the existence of a specific virus?
Who received grants for this vaccine without answering these questions on or before November 22, 2020?
Was it necessary to verify these findings before approving grants for the vaccine?
Is it justified?
Is the vaccine safe for human beings?
Is the vaccine effective for human beings?
Should the mentioned vaccine be approved for human use on or after November 22, 2020?
What evidence proves the safety and efficacy of the mentioned vaccine?
Have they answered all questions relevant to this vaccine?
Who was the exact individual or team of individuals who approved the coronavirus vaccine from your resource?
Where are the answers to the questions relevant to the virus and vaccine that were asked on December 2, 2020, before the approval of the specific vaccine?


What must happen if they are not able to answer these relevant questions?
On or before December 7, 2020, you need to answer these questions or the full team must resign.
All states must be reminded to not use this vaccine until all questions are answered.

What have previous research findings been?
The Tuskegee Syphilis Study still haunts many Americans.
On October 14, 2020, there was discussion about this issue in Chicago, Illinois.
In 1974, specific legislation had to be approved to prevent these issues.
As part of the settlement of a class action lawsuit subsequently filed by the NAACP on behalf of study participants and their descendants, the U.S. government paid $10 million ($51.8 million in 2019 dollars) and other resources to surviving participants and surviving family members infected as a consequence of the study. If all questions relevant to these issues are not answered, grant-grabbing scandals will unfold. Marketing a coronavirus vaccine that is actually something else will unfold.

What else must happen?
The FDA is likely to have deliberations on December 10, 2020, and December 17, 2020. Make sure all questions are answered ahead of any process of approval. Do not approve the vaccine if all questions are not answered.

All state public health resources worldwide should circulate an alert. Not all questions have been answered. Do not use the vaccine until all questions are answered.

Vaccine Issues on or after December 13, 2020
On December 10, 2020, FDA advisers recommended the authorization of the Pfizer COVID-19 vaccine without getting answers to relevant questions: Is this justified?
No.

Why must this process be compared with the Tuskegee Syphilis Study?
The Tuskegee Syphilis Study end date: 1972 still haunts many Americans. This became a scandal while a similar process was used. Not all questions were answered. Similarly, on or after December 12, 2020, the current situation can become a scandal if all questions are not answered by those who have the responsibility to do so before circulating the vaccine.

You are given a vial and told that it is an mRNA vaccine: How do you prove this is really an mRNA vaccine?

How do you scientifically prove that the mRNA vaccine is really generating specific antibodies to this specific virus?

Is this a real vaccine or a saline shot?

Does the vial contain the mentioned ingredients?

Does the vial contain the right concentration of the vaccine?

How did you verify the facts?

Are they falsely claiming the vaccine is more than 94% effective?

Did Pfizer unfairly grab 1 billion dollars for research that could be completed for less than 50,000 dollars?

Do you know that the emergency use authorization before the final-stage testing of a vaccine is complete is not justified?

What are examples of dummy shots and placebos?

Is it justified to approve any vaccine without getting answers to the relevant questions?

Who has the responsibility to remind Pfizer, Moderna, AstraZeneca, and similar entities to answer questions relevant to the vaccine before vaccine circulation?

Is there a focus on public service or an effort to grab research grants and millions in vaccine money without answering the relevant questions?

If you give a placebo or saline shot to a person, there will not be any adverse reaction: Does that mean the ingredients of the vaccine are genuine?

Who has the responsibility to prove that the vial provided has an mRNA vaccine? How do you prove this is really an mRNA vaccine?

Who has responsibility to prove scientifically that this mRNA vaccine is really generating specific antibodies to this specific virus?

What must happen after December 13, 2020, relevant to these issues?
The offices of attorney general of all states: If these questions are not answered ahead of time, remind the vaccine manufacturers of the impending FDA complaints of consumer fraud.

On or after December 13, 2020, replace the head of the FDA and 17 of the 22 members of the Vaccines and Related Biological Products Advisory Committee with new nominees who can answer these questions.

The Vaccines and Related Biological Products Advisory Committee should hold a virtual meeting or deliberations via the Internet that includes the participation of all state attorney generals or their representatives. The meeting must proceed under Chapter 410 Public Health statute of the Illinois compiled statutes or the equivalent. All states must hold similar meetings under the equivalent of Chapter 410 Public Health statute of the Illinois compiled statutes. We may need to have global public health statutes relevant to these issues. A question-and-answer presentation will be appreciated, as circulated by Doctor Asif Qureshi.

Last Updated: December 13, 2020 Last Updated: May 25, 2022